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A study investigated the apoptosis-inducing effect of an oxidative analogue of gambogic acid (GA) on the human hepatocellular carcinoma cell line HepG2 and explore the related molecular mechanisms.
HepG2 cells were treated with the analogue of GA and the growth inhibition was analysed by MTT assay. The morphological changes in cells were observed under an inverted light microscope and a fluorescence microscope.
In addition, both the cell-cycle arrest and the apoptosis rate were detected by flow cytometry. Western blot was used to evaluate the alteration of protein expression.
The viability of HepG2 cells was markedly inhibited in a concentration-dependent manner and obvious morphological changes were confirmed, including condensed chromatin and reduced volume.
Increased percentage of apoptotic cells was displayed and altered expression level of several apoptosis-associated proteins was obtained.
The newly synthesized analogue of GA exhibited potential anticancer activity, induced remarkable apoptosis in HepG2 cells, probably through the intrinsic mitochondrial pathway, and promised to be a new candidate for future cancer therapy.
go site References:
Mu R, Lu N, Wang J, Yin Y, Ding Y, Zhang X, Gui H, Sun Q, Duan H, Zhang L, Zhang Y, Ke X, Guo Q. An Oxidative Analogue Of Gambogic Acid-Induced Apoptosis Of Human Hepatocellular Carcinoma Cell Line HepG2 Is Involved In Its Anticancer Activity In Vitro.” 2010 January http://www.ncbi.nlm.nih.gov/pubmed/19934761
Herb Name: Gamboge
Other names: Gutta gamba. Gambodia. Garcinia Morella. Gummigutta. Tom Rong.
Scientific name: Garcinia hanburyii
Family: N.O. Guttiferae
Common part used: Gum resin.
There are actually quite a few species from which the essential Gamboge is extracted. The tree Garcinia Hanburyii can grow as high as fifteen meters, and can be thirty centimeters thick, or more.
The method of extraction of the resin is by making incisions in the bark of the tree. Alternatively it is also possible to break off leaves or shoots of the tree and let the plant’s juices run. Whichever method is used, the juice is collected in vessels (usually hollow bamboos). This takes place between June and October, in the time of the great monsoon rains. The trees require maturing and cannot be tapped before they are ten years old. The finished product is very similar to gums extracted from the Acacia.
Gamboge has no tradition in Europe, and was certainly completely unknown until the sixteenth century. The Dutch then began to trade in it with other countries in Europe.
Gamboge is brightly colored and is a reliable, bright orange dye. It is completely colorless and odorless, but can display acidic qualities if held in the mouth. It is an extremely potent hydragogue, and is used in treating dropsy. It can also be used to lower the blood pressure.
However, it cannot be administered pure, as it is likely to induce severe vomiting and stomach ache – a strong enough dose can even cause death. However, when used to supplement other medicinal compounds, it is highly effective, and can be used to eradicate the persistent tapeworm infection. However it cannot be stressed too much that any use of this drug must be cautious.
Herb Name: Gamboge
Other Names: yellow Mangosteen
Latin Name: Garcinia tinctoria
Common parts Used: fruit, bark, seed
Gamoge us also know as yellow mangosteen. It is a medium sized tree having large leaves. Gamoge belongs to the family Clusiaceae. Gamboge is yellow berry fruit.
The fruit is considerd to tasty, sometime it is sour too. It is yellow in color but there are other kind of the plant which have purple and some other color of fruits. It is used as substitute for tamarind in cooking. Fruit juice and bark is also used to make dyes.
The fruit is considered to be a good source of vitamin C. it is used as heart tonic. The fruit extract may have the ability to convert excess calories of body in fat. It is also used to suppress appetite. It is also considered energetic. It Is also recommended for the people who are on diet and exercise. The fruit should be not taken in excess.